IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma
• The XMG1.2 antibody is a neutralizing antibody
• Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine
• IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells
• Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma
• Glycosylation of IFN gamma does not affect its biological activity
• Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes
• This can be used at less than or equal to 0.6 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm)
• Most instruments will require a 685 LP mirror and 710/20 filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
• IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
• IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
• On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
• The biological activity of the IFN gamma homodimer is highly species specific
• Human IFN gamma does not show cross-reactivity with mouse
• IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
• IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
• Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
• IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
• IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
• IFN gamma gene mutations are associated with aplastic anemia.