58-044-182

CD44 Monoclonal Antibody (IM7), Alexa Fluor™ 532, eBioscience™, Invitrogen™

Manufacturer: LIFE TECHNOLOGIES

Select a Size

Pack Size SKU Availability Price
Each of 1 58-044-182-Each-of-1 In Stock ₹ 31,913.88

58-044-182 - Each of 1

₹ 31,913.88

In Stock

Quantity

1

Base Price: ₹ 31,913.88

GST (18%): ₹ 5,744.498

Total Price: ₹ 37,658.378

Antigen

CD44

Classification

Monoclonal

Concentration

0.2 mg/ml

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P15379, P16070

Host Species

Rat

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human, Mouse

Product Type

Antibody

Isotype

IgG2b κ

Applications

Flow Cytometry

Clone

IM7

Conjugate

Alexa Fluor 532

Gene

CD44

Gene Symbols

Cd44

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

12505, 960

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

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Description

  • Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1)
  • CD44 is expressed by hematopoietic and non-hematopoietic cells
  • Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44
  • CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component
  • Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This IM7 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This may be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm
  • This cannot be used with the Yellow-Green laser (561 nm)
  • We recommend using a 560/14 band pass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast
  • Several isoforms of CD44 exist, including the predomit CD44H isoform detected in many normal tissues
  • CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration
  • CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing
  • CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential
  • CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes
  • Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events
  • Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined
  • Alternative splicing is the basis for the structural and functional diversity of the CD44 protein
  • Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus
  • CD44 also may be related to tumor metastasis formation.

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LIFE TECHNOLOGIES

58-044-182

--


Antigen:
CD44

Classification:
Monoclonal

Concentration:
0.2 mg/ml

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P15379, P16070

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Human, Mouse

Product Type:
Antibody

Isotype:
IgG2b κ

Applications:
Flow Cytometry

Clone:
IM7

Conjugate:
Alexa Fluor 532

Gene:
CD44

Gene Symbols:
Cd44

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
12505, 960

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Img

Invitrogen

58-045-282

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Antigen:
CD45R (B220)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide, pH 7.2

Gene Accession No.:
P06800, P08575

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Human, Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin)

Clone:
RA3-6B2

Conjugate:
Alexa Fluor 532

Gene:
PTPRC

Gene Symbols:
PTPRC

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
19264, 5788

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Img

Invitrogen

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Antigen:
CD45.2

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P06800

Host Species:
Mouse

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

Applications:
Flow Cytometry

Clone:
104

Conjugate:
Alexa Fluor 532

Gene:
Ptprc

Gene Symbols:
Ptprc

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
19264

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

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Fischer Scientific

58-047-46007

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Purification Method:
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Form:
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