FceR1 alpha Monoclonal Antibody (MAR-1), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability
• Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE
• Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits
• The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM)
• The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions
• Applications Reported: This MAR-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MAR-1 antibody has been tested by flow cytometric analysis of mouse bone marrow-derived mast cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• PE-eFluor™ 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
• We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red™)
• Please make sure that your instrument is capable of detecting this fluorochome
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE
• Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one β and two additional subunits
• The latter two are required for signal transduction activity
• The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response
• When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released
• The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma
• The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.