CX3CR1 Monoclonal Antibody (2A9-1), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family
• CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g
• CD8+, CD4+, and gamma/delta), and monocytes
• In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia
• Little to no CX3CR1 surface expression can be detected on B cells and granulocytes
• Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes
• The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B
• Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 2A9-1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
• We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
•  Please make sure that your instrument is capable of detecting this fluorochrome
• CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection
• The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively
• The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1
• Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine
• CX3CR1 mediates leukocyte migration an d adhesion
• CX3CR1 is expressed in a variety of human tissues and cell lines.