CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa
• This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets
• It has been reported that CD38 binds to CD31
• Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction
• Applications Reported: This WM/59 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This WM/59 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at less than or equal to 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• SB-4400) to minimize any non-specific polymer interactions
• CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases
• CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells
• N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions
• CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis
• It is one of key regulatory molecules in vascular system.