CD42b Monoclonal Antibody (HIP1), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb
• Applications Reported: The HIP1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HIP1 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor
• It interacts with extracellular matrix as well as with adhesion molecules
• CD42b expression and activation seems to be regulated by VEGF and PDGF
• CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface
• CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin
• The extracellular domain of CD42b by its interactions also contributes to metastasis
• Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow
• Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.