CD45RO Monoclonal Antibody (UCHL1), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45
• CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes
• CD22 is a ligand for CD45RO
• Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells
• Applications Reported: This UCHL1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This UCHL1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (1.0 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein
• CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
• CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains
• CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells
• Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.