CD223 (LAG-3) Monoclonal Antibody (3DS223H), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3)
• LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4
• LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation
• Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells
• CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells
• Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells)
• This 3DS223H antibody will recognize a formaldehyde-fixed epitope
• Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4
• LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation
• Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells
• CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells
• Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).