MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s)
• It detects a polymorphic determit present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes
• The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes
• Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye- conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
• SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells
• Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells
• The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.