CD21 Monoclonal Antibody (HB5), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The HB5 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein
• CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV)
• CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation
• Applications Reported: This HB5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HB5 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
• SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen
• CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens
• CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to follicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions
• A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands
• CD21 functions as receptor for C3d, C3dg and iC3b complement components, for EBV and for IFNalpha
• CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation
• Genetic variations in the CD21 gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9)
• Alternatively, spliced transcript variants encoding different isoforms of CD21 have been found.