63-080-942

CD80 (B7-1) Monoclonal Antibody (2D10.4), Super Bright™ 600, eBioscience™, Invitrogen™

Manufacturer: eBioscience

Select a Size

Pack Size SKU Availability Price
Each of 1 63-080-942-Each-of-1 In Stock ₹ 36,961.92

63-080-942 - Each of 1

₹ 36,961.92

In Stock

Quantity

1

Base Price: ₹ 36,961.92

GST (18%): ₹ 6,653.146

Total Price: ₹ 43,615.066

Antigen

CD80 (B7-1)

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P33681

Gene Symbols

CD80

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

941

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

2D10.4

Conjugate

Super Bright 600

Gene

CD80

Gene Alias

Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily
  • CD80 is expressed by activated B cells, macrophages and dendritic cells
  • In addition, activated T cells express this antigen
  • CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
  • The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
  • Applications Reported: This 2D10.4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of Daudi cell line
  • This can be used at 5 μL (0.5 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
  • We recommend using a 610/20 bandpass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
  • SB-4401) to minimize any non-specific polymer interactions
  • Please refer to the datasheet for Super Bright Staining Buffer for more information
  • CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
  • Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
  • CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
  • The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
  • Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Compare Similar Items

Show Difference

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eBioscience

63-080-942

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Antigen:
CD80 (B7-1)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P33681

Gene Symbols:
CD80

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
941

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
2D10.4

Conjugate:
Super Bright 600

Gene:
CD80

Gene Alias:
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

Img

R&D Systems

63-081

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Antigen:
__

Classification:
__

Concentration:
__

Formulation:
__

Gene Accession No.:
__

Gene Symbols:
__

Purification Method:
__

Regulatory Status:
__

Gene ID (Entrez):
__

Content And Storage:
Store at -20°C

Form:
__

Applications:
__

Clone:
__

Conjugate:
__

Gene:
__

Gene Alias:
__

Host Species:
__

Quantity:
1 mg

Primary or Secondary:
__

Target Species:
__

Product Type:
__

Isotype:
__

Img

Fischer Scientific

63-086-941

--


Antigen:
CD86 (B7-2)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P42081

Gene Symbols:
CD86

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
942

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
IT2.2

Conjugate:
Super Bright 600

Gene:
CD86

Gene Alias:
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2

Host Species:
Mouse

Quantity:
25 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG2b κ

Img

Fischer Scientific

63-086-942

--


Antigen:
CD86 (B7-2)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P42081

Gene Symbols:
CD86

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
942

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
IT2.2

Conjugate:
Super Bright 600

Gene:
CD86

Gene Alias:
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG2b κ