IgE Monoclonal Antibody (Ige21), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody IgE21 recognizes the immunoglobulin E class of antibodies
• The natural levels of IgE in the serum are quite low, comprising only a small portion of the total immunoglobulin
• IgE plays a role in allergy responses by binding to mast cells and basophils through the Fc epsilon receptor I which results in the release of histamine and other molecules
• The IgE is produced by terminally differentiated plasma B cells and basophils
• Like all immunoglobulins, IgE is expressed as a soluble molecule as well as a membrane bound form
• IgE21 recognizes both forms
• Applications Reported: This IgE21 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This IgE21 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• IgE is one of the five classes of immunoglobulins found in mammals
• Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains
• IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells
• Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations
• IgE is also functionally important for immunity against parasites.