CD279 (PD-1) Monoclonal Antibody (RMP1-30), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C
• MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance
• W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains
• Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey
• Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This W6/32 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (1.0 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat
• SB-4400) to minimize any non-specific polymer interactions
• Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC)
• T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC
• PD-1 can bind two different but related ligands, PDL-1 and PDL-2
• Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of &quotedanger signals&quote such as LPS or other molecules associated with bacteria or other pathogens
• Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases
• Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.