CD184 (CXCR4) Monoclonal Antibody (2B11), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM
• It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA
• IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells
• Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat
• SB-4400) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Protect this vial and stained samples from light
• CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells
• CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus
• In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells
• CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation
• CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels
• Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells
• In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development
• CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes
• Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells
• The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.