CD138 (Syndecan-1) Monoclonal Antibody (DL-101), Super Bright™ 600, eBioscience™, Invitrogen™

Description

• The DL-101 monoclonal antibody reacts with human CD138, also known as syndecan-1
• CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components
• CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells
• This DL-101 antibody has been pre-titrated and tested by flow cytometric analysis of the U266 cell line
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
• We recommend using a 610/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No
• SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes
• CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury
• It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells
• Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics
• Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.