CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA)
• CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer
• While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation
• Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors
• CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin
• CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface
• CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51
• CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin
• The CD61 protein product is composed of an alpha chain and a beta chain
• A given chain may combine with multiple partners resulting in different integrins
• CD61 is found along with the alpha IIb chain in platelets
• Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling
• Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.