CD80 (B7-1) Monoclonal Antibody (16-10A1), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The GL1 monoclonal antibody reacts with mouse CD86, an ∽80 kDa surface receptor also known as B7-2
• CD86 and CD80 are members of the B7 family of costimulatory molecules
• CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors
• CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells
• In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4)
• This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production
• The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response
• Applications Reported: This GL1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This GL1 antibody has been tested by flow cytometric analysis
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
• Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
• CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
• The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
• Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.