CD48 Monoclonal Antibody (HM48-1), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit
• The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106)
• This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils
• CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1)
• Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
• We recommend using a 710/50 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• SB-4400) to minimize any non-specific polymer interactions
• CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others
• These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion
• CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2
• 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation
• Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases
• CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium
• CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2
• 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.