Phospho-SMAD2 (Ser245, Ser250) Recombinant Rabbit Monoclonal Antibody (2H24L4), Invitrogen™

Description

• This antibody is predicted to react with Monkey, Pig, Rabbit Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
• The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
• Then, individual clones are screened to select the best candidates for production
• The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
• SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation
• SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling
• Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin
• In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors
• The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4
• The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors
• SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin
• Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.