7067841

Mouse CD45 Antibody, R&D Systems™

Rat Monoclonal Antibody has been used in 8 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD45

Dilution

Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Immunoprecipitation, Complement-dependent Cytotoxicity, Immunocytochemistry 3-25 ug/mL, CyTOF-reported

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene ID (Entrez)

5788

Immunogen

Mouse thymus and spleen cells

Primary or Secondary

Primary

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity

Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15).

Clone

30-F11

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Cytotoxicity Assay, Immunocytochemistry

Conjugate

Unconjugated

Host Species

Rat

Target Species

Mouse

Gene Alias

B220, CD45, CD45 antigen, CD45R, EC 3.1.3.48, LCA, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols

PTPRC

Isotype

IgG2b

Purification Method

Protein A or G purified from hybridoma culture supernatant

Content And Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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Description

  • CD45 Monoclonal specifically detects CD45 in Mouse samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Complement-dependent Cytotoxicity, Immunocytochemistry, CyTOF-reported.

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7067841

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Antigen:
CD45

Dilution:
Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Immunoprecipitation, Complement-dependent Cytotoxicity, Immunocytochemistry 3-25 ug/mL, CyTOF-reported

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene ID (Entrez):
5788

Immunogen:
Mouse thymus and spleen cells

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15).

Clone:
30-F11

Applications:
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Cytotoxicity Assay, Immunocytochemistry

Conjugate:
Unconjugated

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
B220, CD45, CD45 antigen, CD45R, EC 3.1.3.48, LCA, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols:
PTPRC

Isotype:
IgG2b

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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Antigen:
Galectin-3

Dilution:
Western Blot 0.2 ug/mL, Simple Western 10 ug/mL, Immunohistochemistry 1-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene ID (Entrez):
3958

Immunogen:
E. coli-derived recombinant human Galectin-3 Ala2-Ile250 Accession # P17931.5

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human Galectin-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human Galectin-1, 2, 4, 7, 8, or 10 is observed.

Clone:
194805

Applications:
Western Blot, Immunohistochemistry, ELISA

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Alias:
AGE-R3, Carbohydrate-binding protein 35, CBP 35,35 kDa lectin, CBP35, Gal-3, GAL3, galactin-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBPCBP35, galectin-3, Galectin3, GALIG, IgE-binding protein, L29, L31, L-31, Laminin-binding protein, Lectin L-29, lectin, galactoside-binding, soluble, 3, LGALS2, LGALS3, Mac-2, Mac-2 antigen, MAC2GAL3

Gene Symbols:
LGALS3

Isotype:
IgG2b

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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Antigen:
CD8

Dilution:
Flow Cytometry 0.25 ug/10^6 cells, Immunoprecipitation, Cell depletion, Inhibition of T Cell Function, Immunocytochemistry 8-25 ug/mL, CyTOF-ready

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene ID (Entrez):
925

Immunogen:
Mouse thymus or spleen

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects both the alpha and alpha ' chains of mouse CD8 (1).

Clone:
53-6.7

Applications:
Flow Cytometry, Immunoprecipitation, Depletion Assay, Inhibition Assays, Immunocytochemistry

Conjugate:
Unconjugated

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
CD8, CD8A, CD8a molecule, LEU2, p32

Gene Symbols:
CD8A

Isotype:
IgG2a

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

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Antigen:
DPPIV/CD26

Dilution:
Western Blot 2 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative

Gene ID (Entrez):
1803

Immunogen:
Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 Asp34-Pro766 Accession # Q53TN1

Primary or Secondary:
Primary

Reconstitution:
Reconstitute at 0.5 mg/mL in sterile PBS.

Test Specificity:
Detects human DPPIV/CD26 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse DPPIV is observed.

Clone:
222113

Applications:
Western Blot, Flow Cytometry, CyTOF, ELISA

Conjugate:
Unconjugated

Host Species:
Rat

Target Species:
Human

Gene Alias:
ADABP, ADCP-2, ADCP2DPP IV, Adenosine deaminase complexing protein 2TP103, CD26, CD26 antigen, CD26T-cell activation antigen CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, dipeptidylpeptidase 4, dipeptidyl-peptidase 4, dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2), DPP4, DPPIV, EC 3.4.14.5

Gene Symbols:
DPP4

Isotype:
IgG2a

Purification Method:
Protein A or G purified from hybridoma culture supernatant

Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.