MHC Class II (I-A) Monoclonal Antibody (NIMR-4), FITC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens
• Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells
• MHC Class II molecules are important for the selection of the TCR repertoire during the processes of positive and negative selection
• MHC Class II expression is required for the positive selection of CD4+ T cells
• Applications Reported: This NIMR-4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This NIMR-4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains
• Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes
• First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides
• Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside
• MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells
• CD4 interacts with non-polymorphic residues of MHC class II
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.