7068313
Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Ly-6A/E (Sca-1)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Gene Symbols
Ly6a, Ly6e
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
D7
Applications
Flow Cytometry
Conjugate
PE
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P05533, Q64253
Gene ID (Entrez)
110454, 17069
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins
- D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells
- Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others
- Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype
- The Ly-6 family is involved in regulation and function of T cell activation
- Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset
- Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Filtration: 0.2 μm post-manufacturing filtered
- Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family
- It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice
- Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC
- Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen
- Sca-1 may be involved in the regulation of B and T cell activation.
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Antigen: Ly-6A/E (Sca-1)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Gene Symbols: Ly6a, Ly6e
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: D7
Applications: Flow Cytometry
Conjugate: PE
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P05533, Q64253
Gene ID (Entrez): 110454, 17069
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Ly-6A/E (Sca-1)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Gene Symbols:
Ly6a, Ly6e
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
D7
Applications:
Flow Cytometry
Conjugate:
PE
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P05533, Q64253
Gene ID (Entrez):
110454, 17069
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD274 (PD-L1, B7-H1)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols: Cd274
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: MIH1
Applications: Flow Cytometry
Conjugate: PE
Host Species: Mouse
Target Species: Human
Gene Accession No.: Q9NZQ7
Gene ID (Entrez): 29126
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD274 (PD-L1, B7-H1)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols:
Cd274
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
MIH1
Applications:
Flow Cytometry
Conjugate:
PE
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
Q9NZQ7
Gene ID (Entrez):
29126
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: IgD
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Gene Symbols: Ighd
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: 11-26c (11-26)
Applications: Flow Cytometry
Conjugate: PE
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P01881
Gene ID (Entrez): 380797
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
IgD
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Gene Symbols:
Ighd
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
11-26c (11-26)
Applications:
Flow Cytometry
Conjugate:
PE
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P01881
Gene ID (Entrez):
380797
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: MHC Class I (H-2Kd/H-2Dd)
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2-D1; H2-DC1-beta transplantation antigen protein; H2DD; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2K1; H2-K1; H2KD; histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein
Gene Symbols: H2-D1, H2-K1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: 34-1-2S
Applications: Flow Cytometry
Conjugate: FITC
Host Species: Mouse
Target Species: Mouse
Gene Accession No.: P01895, P01896, P01897, P01899, P01900, P01901, P03991, P04223, P14426, P14427, P14428
Gene ID (Entrez): 14964, 14972
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
MHC Class I (H-2Kd/H-2Dd)
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2-D1; H2-DC1-beta transplantation antigen protein; H2DD; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2K1; H2-K1; H2KD; histocompatibility 2, D region locus 1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein
Gene Symbols:
H2-D1, H2-K1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
34-1-2S
Applications:
Flow Cytometry
Conjugate:
FITC
Host Species:
Mouse
Target Species:
Mouse
Gene Accession No.:
P01895, P01896, P01897, P01899, P01900, P01901, P03991, P04223, P14426, P14427, P14428
Gene ID (Entrez):
14964, 14972
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody