IL-25R (IL-17RB) Monoclonal Antibody (MUNC33), PE, eBioscience™, Invitrogen™

Description

• Description: This MUNC33 monoclonal antibody reacts with mouse interleukin-25 receptor (IL-25R), also known as IL-17RB
• IL-25R is a 55 kDa transmembrane protein that dimerizes with IL-17RA to form the functional receptor for IL-25 (IL-17E)
• IL-25R is detectable in various tissues, including the lung and gastrointestinal tract and exists as both membrane bound and soluble form
• IL-25/IL-25R interaction has been shown to activate the NF-kB, MAPK, and JNK pathways as well as gene transcription by STAT6, GATA-3, and NFATc1
• IL-25R signals in T helper 2 (Th2) cells, type 2 innate lymphoid (ILC2) cells and some iNKT cells to promote differentiation and effector function
• Whereas IL-25-deficient mice have an impaired Th2 response to parasitic helminth infection, elevated expression of IL-25 and IL-25R is observed in patients with chronic asthma and atopic dermatitis
• Applications Reported: This MUNC33 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MUNC33 antibody has been tested by flow cytometirc analysis of mouse lymph node cells or IL-25R-transfected cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified
• These are termed as IL-17B, IL-17C (1) and IL-17E
• IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth
• IL-17B, C, and E like IL-17 are able to induce proinflammatory responses
• However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist
• Receptor for IL-17B and IL-17E has been independently isolated and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively
• IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8
• IL-17Rh1 mRNA is highly expressed in Kidney and liver and at moderate level in small intestine, colon, and brain.