Myeloperoxidase (MPO) Monoclonal Antibody (MPO455-8E6), FITC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody MPO455-8E6 recognizes myeloperoxidase (MPO), a protein within the azurophilic granules of myeloid cells
• MPO is a multimeric protein comprised of two 55 kDa subunits and two 15 kDa subunits
• The larger subunits associate with a heme protein resulting in a greenish color
• As an enzyme, MPO breaks down hydrogen peroxide and oxidizes tyrosine
• The products of this reaction, hypochlorous acid and tyrosyl radical, cause the cytotoxic and killing effects characteristic of neutrophils
• Myeloperoxidase is important in the diagnosis of some cancers and increases in serum levels have been shown to correlate with cardiac events
• Applications Reported: This MPO455-8E6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This MPO455-8E6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824)
• For best results, whole blood should first be stained with antibodies to surface antigens then treated with 1X RBC lysis buffer (cat
• 00-4333) to lyse erythrocytes
• Then, for intracellular staining for MPO, cells should be fixed with IC Fixation Buffer (cat
• 00-8222) washed two times with Permeabilization Buffer (cat
• 00-8333) and then incubated with MPO455-8E6 for 30-60 minutes
• After washing, cells may be analyzed on a flow cytometer
• Myeloperoxidase (MPO) is a heme protein that is synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules
• It is produced as a single chain precursor and subsequently cleaved into a light and heavy chain
• The mature MPO is a tetramer composed of 2 light chains and 2 heavy chains
• This enzyme is responsible for producing hypohalous acids that are central to the microbicidal activity of neutrophils
• Diseases that are associated with MPO include Myeloperoxidase Deficiency and Alzheimer's Disease, Familial, 1.