7068798
SIGLEC H Monoclonal Antibody (eBio440c), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
SIGLEC H
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein
Gene Symbols
Siglech
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Siglech
Clone
eBio440c
Applications
Flow Cytometry
Conjugate
PE
Host Species
Rat
Target Species
Mouse
Gene Accession No.
0
Gene ID (Entrez)
233274
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
- Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
- Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
- To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
- The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
- It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6
- Applications Reported: This eBio440c antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBio440c antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse
- Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses
- Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif)
- To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction
- The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG)
- It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.
Compare Similar Items
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Antigen: SIGLEC H
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein
Gene Symbols: Siglech
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Siglech
Clone: eBio440c
Applications: Flow Cytometry
Conjugate: PE
Host Species: Rat
Target Species: Mouse
Gene Accession No.: 0
Gene ID (Entrez): 233274
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
SIGLEC H
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein
Gene Symbols:
Siglech
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Siglech
Clone:
eBio440c
Applications:
Flow Cytometry
Conjugate:
PE
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
0
Gene ID (Entrez):
233274
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD33
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Gene Symbols: CD33
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: CD33
Clone: WM-53 (WM53)
Applications: Flow Cytometry
Conjugate: PE
Host Species: Mouse
Target Species: Human
Gene Accession No.: P20138
Gene ID (Entrez): 945
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD33
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Gene Symbols:
CD33
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
CD33
Clone:
WM-53 (WM53)
Applications:
Flow Cytometry
Conjugate:
PE
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P20138
Gene ID (Entrez):
945
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD33
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Gene Symbols: CD33
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: CD33
Clone: HIM3-4
Applications: Flow Cytometry
Conjugate: PE
Host Species: Mouse
Target Species: Human
Gene Accession No.: P20138
Gene ID (Entrez): 945
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD33
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Gene Symbols:
CD33
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
CD33
Clone:
HIM3-4
Applications:
Flow Cytometry
Conjugate:
PE
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P20138
Gene ID (Entrez):
945
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD34
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme R1; AU040960; CD34; CD34 antigen; CD34 molecule; CDC34; cell division cycle 34; cell division cycle 34 homolog; cluster designation 34; cluster of differentiation antigen 34; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
Gene Symbols: Cd34
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Cd34
Clone: 1H6
Applications: Flow Cytometry
Conjugate: PE
Host Species: Mouse
Target Species: Canine
Gene Accession No.: Q28270
Gene ID (Entrez): 415130
Isotype: IgG1
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD34
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
(E3-independent) E2 ubiquitin-conjugating enzyme R1; AU040960; CD34; CD34 antigen; CD34 molecule; CDC34; cell division cycle 34; cell division cycle 34 homolog; cluster designation 34; cluster of differentiation antigen 34; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
Gene Symbols:
Cd34
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Cd34
Clone:
1H6
Applications:
Flow Cytometry
Conjugate:
PE
Host Species:
Mouse
Target Species:
Canine
Gene Accession No.:
Q28270
Gene ID (Entrez):
415130
Isotype:
IgG1
Purification Method:
Affinity chromatography
Product Type:
Antibody