7069180
TNF alpha Polyclonal Antibody, Biotin, eBioscience™, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
TNF alpha
Applications
ELISA
Conjugate
Biotin
Host Species
Rabbit
Target Species
Mouse, Rat
Gene Accession No.
P06804, P16599
Gene ID (Entrez)
21926, 24835
Isotype
IgG
Purification Method
Affinity chromatography
Product Type
Antibody
Concentration
0.5 mg/mL
Classification
Polyclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols
Tnf
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Tnf
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Description
- Description: The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha
- Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells
- A 26 kDa form is expressed as a membrane-bound molecule
- TNF alpha is cytolytic and plays an important role in immune regulation
- Bioactive TNF alpha exists as a homotrimer
- Applications Reported: This polyclonal antibody has been reported for use in ELISA and ELISPOT
- Applications Tested: The biotinylated rabbit anti-mouse/rat TNF alpha polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the purified anti-mouse/rat TNF alpha monoclonal antibody, clone TN3.19 (14-7423) for capture and recombinant mouse TNF alpha (14-8321) or rat TNF alpha (14-8321) as the standard
- A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 - 2 μg/mL
- A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL (mouse TNF alpha) or 5000 - 30 pg/mL (rat TNF alpha) should be included in each ELISA plate
- Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF alpha receptor type I or II
- Filtration: 0.2 μm post-manufacturing filtered
- TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
- This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
- TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
- TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
- Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
- Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
- TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
- This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
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Antigen: TNF alpha
Applications: ELISA
Conjugate: Biotin
Host Species: Rabbit
Target Species: Mouse, Rat
Gene Accession No.: P06804, P16599
Gene ID (Entrez): 21926, 24835
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Concentration: 0.5 mg/mL
Classification: Polyclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols: Tnf
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Tnf
Antigen:
TNF alpha
Applications:
ELISA
Conjugate:
Biotin
Host Species:
Rabbit
Target Species:
Mouse, Rat
Gene Accession No.:
P06804, P16599
Gene ID (Entrez):
21926, 24835
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody
Concentration:
0.5 mg/mL
Classification:
Polyclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols:
Tnf
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Tnf
Antigen: TNF alpha
Applications: ELISA
Conjugate: Biotin
Host Species: Mouse
Target Species: Human
Gene Accession No.: P01375
Gene ID (Entrez): 7124
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols: Tnf
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Tnf
Antigen:
TNF alpha
Applications:
ELISA
Conjugate:
Biotin
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P01375
Gene ID (Entrez):
7124
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols:
Tnf
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Tnf
Antigen: CD266 (TWEAK Receptor)
Applications: Flow Cytometry
Conjugate: Biotin
Host Species: Mouse
Target Species: Human, Mouse
Gene Accession No.: Q9CR75, Q9NP84
Gene ID (Entrez): 27279, 51330
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AI255180; C87282; CD266; FGF-inducible 14; Fgfrp2; fibroblast growth factor regulated protein 2; fibroblast growth factor-inducible immediate-early response protein 14; Fibroblast growth factor-regulated protein 2; FN14; HPIP; TNF receptor superfamily member 12A; TNFRSF12A; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; TweakR; TWEAK-R; Tweak-receptor; type I transmembrane protein Fn14
Gene Symbols: TNFRSF12A
Primary or Secondary: Primary
Content And Storage: 4° C, do not freeze
Gene: TNFRSF12A
Antigen:
CD266 (TWEAK Receptor)
Applications:
Flow Cytometry
Conjugate:
Biotin
Host Species:
Mouse
Target Species:
Human, Mouse
Gene Accession No.:
Q9CR75, Q9NP84
Gene ID (Entrez):
27279, 51330
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AI255180; C87282; CD266; FGF-inducible 14; Fgfrp2; fibroblast growth factor regulated protein 2; fibroblast growth factor-inducible immediate-early response protein 14; Fibroblast growth factor-regulated protein 2; FN14; HPIP; TNF receptor superfamily member 12A; TNFRSF12A; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; TweakR; TWEAK-R; Tweak-receptor; type I transmembrane protein Fn14
Gene Symbols:
TNFRSF12A
Primary or Secondary:
Primary
Content And Storage:
4° C, do not freeze
Gene:
TNFRSF12A
Antigen: CD266 (TWEAK Receptor)
Applications: Flow Cytometry
Conjugate: Biotin
Host Species: Mouse
Target Species: Human, Mouse
Gene Accession No.: Q9CR75, Q9NP84
Gene ID (Entrez): 27279, 51330
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AI255180; C87282; CD266; FGF-inducible 14; Fgfrp2; fibroblast growth factor regulated protein 2; fibroblast growth factor-inducible immediate-early response protein 14; Fibroblast growth factor-regulated protein 2; FN14; HPIP; TNF receptor superfamily member 12A; TNFRSF12A; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; TweakR; TWEAK-R; Tweak-receptor; type I transmembrane protein Fn14
Gene Symbols: TNFRSF12A
Primary or Secondary: Primary
Content And Storage: 4° C, do not freeze
Gene: TNFRSF12A
Antigen:
CD266 (TWEAK Receptor)
Applications:
Flow Cytometry
Conjugate:
Biotin
Host Species:
Mouse
Target Species:
Human, Mouse
Gene Accession No.:
Q9CR75, Q9NP84
Gene ID (Entrez):
27279, 51330
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AI255180; C87282; CD266; FGF-inducible 14; Fgfrp2; fibroblast growth factor regulated protein 2; fibroblast growth factor-inducible immediate-early response protein 14; Fibroblast growth factor-regulated protein 2; FN14; HPIP; TNF receptor superfamily member 12A; TNFRSF12A; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; TweakR; TWEAK-R; Tweak-receptor; type I transmembrane protein Fn14
Gene Symbols:
TNFRSF12A
Primary or Secondary:
Primary
Content And Storage:
4° C, do not freeze
Gene:
TNFRSF12A