7069678
Nestin Monoclonal Antibody (10C2), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Nestin
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Gene Symbols
Nes
Primary or Secondary
Primary
Content And Storage
4° C
Gene
Nes
Clone
10C2
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human
Gene Accession No.
P48681
Gene ID (Entrez)
10763
Isotype
IgG1
Purification Method
Affinity chromatography
Product Type
Antibody
Description
- Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614)
- Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system
- Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential
- Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP
- Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors
- No cross-reactivity to rodent nestins has been observed using the 10C2 antibody
- Applications Reported: This 10C2 antibody has been reported for use in immunocytochemistry, microscopy, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis
- Applications Tested: This 10C2 antibody has been tested by western blot on SDS-reduced and non-reduced lysates from U251 cells and can be used at less than or equal to 5 μg/mL
- The 10C2 antibody has also been tested by immunocytochemistry on formaldehyde-fixed and permeabilized SKNSH cells and can be used at less than or equal to 5 μg/mL
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells
- Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells
- Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors
- Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells
- Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
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Antigen: Nestin
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Gene Symbols: Nes
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Nes
Clone: 10C2
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P48681
Gene ID (Entrez): 10763
Isotype: IgG1
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Nestin
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Gene Symbols:
Nes
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Nes
Clone:
10C2
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P48681
Gene ID (Entrez):
10763
Isotype:
IgG1
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Collagen IV
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: [a]3(IV); [a]4(IV); alpha 1 type IV collagen; alpha type IV collagen basement membrane; alpha1(IV) collagen; alpha-2 collagen type IV; alpha-3 type IV collagen; alpha3(IV); Arresten; Bru; BSVD; CA44; Canstatin; Col4a1; Col4a-1; COL4A1 NC1 domain; Col4a2; Col4a-2; COL4A3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha 1 chain; collagen type IV alpha 2; collagen type IV alpha 2 chain; collagen type IV alpha 3 chain; collagen type IV alpha 4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 2; collagen, type IV, alpha 3; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)44H; Del(8)Bru44H; E130010M05Rik; Goodpasture antigen; ICH; LOW QUALITY PROTEIN: collagen alpha-1(IV) chain; LOW QUALITY PROTEIN: collagen alpha-3(IV) chain; LOW QUALITY PROTEIN: collagen alpha-4(IV) chain; POREN2; procollagen alpha1(IV); procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; RATOR; Raw; retinal anterior wiring; Svc; tumstatin; Type IV Collagen
Gene Symbols: COL4A1, COL4A2, Col4a3, COL4A4, COL4A5
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: __
Clone: 1042
Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Gene Accession No.: P02462, P08572, P29400, P53420, Q01955
Gene ID (Entrez): 1282, 1284, 1285, 1286, 1287
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Collagen IV
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
[a]3(IV); [a]4(IV); alpha 1 type IV collagen; alpha type IV collagen basement membrane; alpha1(IV) collagen; alpha-2 collagen type IV; alpha-3 type IV collagen; alpha3(IV); Arresten; Bru; BSVD; CA44; Canstatin; Col4a1; Col4a-1; COL4A1 NC1 domain; Col4a2; Col4a-2; COL4A3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen type IV alpha 1 chain; collagen type IV alpha 2; collagen type IV alpha 2 chain; collagen type IV alpha 3 chain; collagen type IV alpha 4 chain; collagen type IV alpha3 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 2; collagen, type IV, alpha 3; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Del(8)44H; Del(8)Bru44H; E130010M05Rik; Goodpasture antigen; ICH; LOW QUALITY PROTEIN: collagen alpha-1(IV) chain; LOW QUALITY PROTEIN: collagen alpha-3(IV) chain; LOW QUALITY PROTEIN: collagen alpha-4(IV) chain; POREN2; procollagen alpha1(IV); procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; RATOR; Raw; retinal anterior wiring; Svc; tumstatin; Type IV Collagen
Gene Symbols:
COL4A1, COL4A2, Col4a3, COL4A4, COL4A5
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
__
Clone:
1042
Applications:
Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P02462, P08572, P29400, P53420, Q01955
Gene ID (Entrez):
1282, 1284, 1285, 1286, 1287
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: GFAP
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
Gene Symbols: GFAP
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: GFAP
Clone: GA5
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Chicken, Human, Mouse, Porcine, Rabbit, Rat
Gene Accession No.: P03995, P14136, P47819
Gene ID (Entrez): 100352532, 14580, 24387, 2670, 396562, 419969
Isotype: IgG1
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
GFAP
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
Gene Symbols:
GFAP
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
GFAP
Clone:
GA5
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Chicken, Human, Mouse, Porcine, Rabbit, Rat
Gene Accession No.:
P03995, P14136, P47819
Gene ID (Entrez):
100352532, 14580, 24387, 2670, 396562, 419969
Isotype:
IgG1
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Musashi-1
Concentration: 0.5 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: fb40b12; hypothetical protein LOC541389; m-Msi-1; ms1; msi1; msi1b; Msi1h; Musahi1; musashi homolog 1; Musashi homolog 1(Drosophila); musashi RNA binding protein 1; musashi RNA binding protein 1b; musashi RNA-binding protein 1; musashi1; musashi-1; RNA-binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1 L+35bp; RNA-binding protein Musashi homolog 1 Long; RNA-binding protein Musashi homolog 1b; wu:fb40b12; zgc:103751; zMsi1
Gene Symbols: MSI1
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: MSI1
Clone: 14H1
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Host Species: Rat
Target Species: Human, Mouse, Rat
Gene Accession No.: O43347, Q61474, Q8K3P4
Gene ID (Entrez): 17690, 259272, 4440
Isotype: IgG2a
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Musashi-1
Concentration:
0.5 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
fb40b12; hypothetical protein LOC541389; m-Msi-1; ms1; msi1; msi1b; Msi1h; Musahi1; musashi homolog 1; Musashi homolog 1(Drosophila); musashi RNA binding protein 1; musashi RNA binding protein 1b; musashi RNA-binding protein 1; musashi1; musashi-1; RNA-binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1 L+35bp; RNA-binding protein Musashi homolog 1 Long; RNA-binding protein Musashi homolog 1b; wu:fb40b12; zgc:103751; zMsi1
Gene Symbols:
MSI1
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
MSI1
Clone:
14H1
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Host Species:
Rat
Target Species:
Human, Mouse, Rat
Gene Accession No.:
O43347, Q61474, Q8K3P4
Gene ID (Entrez):
17690, 259272, 4440
Isotype:
IgG2a
Purification Method:
Affinity chromatography
Product Type:
Antibody