7069776

CD135 (Flt3) Monoclonal Antibody (A2F10), PE-Cyanine5, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD135 (Flt3)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

B230315G04; CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK2; Flk-2; FLT3; Flt-3; fms related tyrosine kinase 3; FMS-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Ly72; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; stem cell tyrosine kinase 1; STK1; STK-1; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmfl

Gene Symbols

FLT3

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

FLT3

Clone

A2F10

Applications

Flow Cytometry

Conjugate

PE-Cyanine5

Host Species

Rat

Target Species

Mouse

Gene Accession No.

Q00342

Gene ID (Entrez)

14255

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3
  • A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow
  • Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively
  • Applications Reported: This A2F10 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family
  • The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor
  • The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow
  • Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

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7069776

Rat Monoclonal Antibody...


Antigen:
CD135 (Flt3)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
B230315G04; CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK2; Flk-2; FLT3; Flt-3; fms related tyrosine kinase 3; FMS-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Ly72; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; stem cell tyrosine kinase 1; STK1; STK-1; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmfl

Gene Symbols:
FLT3

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
FLT3

Clone:
A2F10

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine5

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q00342

Gene ID (Entrez):
14255

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7069778

Rat Isotype Control...


Antigen:
Rat IgG2b kappa

Concentration:
0.2 mg/mL

Classification:
__

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Gene Symbols:
__

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
__

Clone:
eB149/10H5

Applications:
Control, Flow Cytometry

Conjugate:
PE-Cyanine5

Host Species:
Rat

Target Species:
Not Applicable

Gene Accession No.:
__

Gene ID (Entrez):
__

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
__

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Antigen:
FOXP3

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID

Gene Symbols:
Foxp3

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Foxp3

Clone:
PCH101

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine5

Host Species:
Rat

Target Species:
Chimpanzee, Cynomolgus Monkey, Human, Non-human Primate, Rhesus Monkey

Gene Accession No.:
Q6U8D7, Q9BZS1

Gene ID (Entrez):
102120882, 50943, 574303, 740909

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
F4/80

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3

Gene Symbols:
ADGRE1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
ADGRE1

Clone:
BM8

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine5

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q61549

Gene ID (Entrez):
13733

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody