TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), PE-Cyanine5, eBioscience™, Invitrogen™

Description

• Description: The GL3 monoclonal antibody reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR bearing T cells, but it does not react with alpha beta TCR
• The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues
• Applications Reported: This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis
• It is recommended to prestain cells with anti-mouse CD16/32 (cat
• 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody
• Applications Tested: This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Light sensitivity: This tandem dye is sensitive photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system
• The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response
• TCR recognition of self-peptides has been linked to autoimmune disease
• Mutant self-peptides have been associated with tumors
• Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome
• Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)
• The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease
• Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP)
• However, a small number of T cells lack both CD4 and CD8 (double negative, DN)
• Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders
• Gamma-delta T cells are primarily found within the epithelium
• They show less TCR diversity and recognize antigens differently than alpha-beta T cells
• Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.