CD16 Monoclonal Antibody (3G8), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: This 3G8 monoclonal antibody reacts with human and non-human primate CD16, which is also known as the low-affinity Fc gamma RIII
• CD16 exists as two distinct isoforms, Fc gamma RIIIA and Fc gamma RIIIB
• In humans, Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as NK cells
• T and B cells do not express this Fc receptor
• Fc gamma RIIIB is also detected on neutrophils as a GPI-anchored form
• Expression of CD16 on lymphocytes and monocytes is similar in non-human primates
• However, while CD16 is not found on neutrophils in macaques and baboons, this receptor is detected on these cells in sooty mangabeys
• Binding of IgG leads to activation of signal transduction pathways, resulting in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation
• This monoclonal antibody has been reported to have several functional activities, including inhibition of cytotoxic ability, activation of cell signaling, and NK cell depletion in vivo
• Moreover, the 3G8 antibody clone has been demonstrated to work on capuchin monkey, chimpanzee, common marmoset, cynomologous monkey, hamadyras baboon, olive baboon, pigtailed macaque, rhesus, and squirrel monkey
• Based on cross-blocking studies 3G8 recognizes the same epitope as CB16
• However, 3G8 and B73.1 antibody clones bind distinct epitopes
• CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections
• This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1
• The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage
• Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia
• Alternatively spliced transcript variants encoding different isoforms have been found for this gene
• Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.