CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit
• The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106)
• This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils
• CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1)
• Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis
• R1-2 has also been reported in in vitro functional assays
• Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins
• Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains
• Alpha4 chain associates with either beta1 or beta7 chain
• It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain
• These ligands included Madcam, VCAM, and fibronectin
• Madcam is known as the principal ligand for integrin alpha4beta7
• Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.