7069848
CD80 (B7-1) Monoclonal Antibody (16-10A1), Functional Grade, eBioscience™, Invitrogen™
Armenian Hamster Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD80 (B7-1)
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with no preservative; pH 7.2
Gene Alias
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
Gene Symbols
CD80
Primary or Secondary
Primary
Content And Storage
4° C
Gene
CD80
Clone
16-10A1
Applications
Flow Cytometry, Functional Assay, Neutralization
Conjugate
Functional Grade
Host Species
Armenian Hamster
Target Species
Canine, Mouse, Porcine
Gene Accession No.
Q00609
Gene ID (Entrez)
12519, 397161, 403765
Isotype
IgG
Purification Method
Affinity chromatography
Product Type
Antibody
Description
- Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily
- CD80 is expressed by macrophages, dendritic cells and activated B cells
- In addition, activated T cells express this antigen
- CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
- The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
- Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis
- 16-10A1 has also been reported in blocking of CD80 to its ligands
- Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Storage and handling: Use in a sterile environment
- Filtration: 0.2 μm post-manufacturing filtered
- Purity: Greater than 90%, as determined by SDS-PAGE
- Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
- Aggregation: Less than 10%, as determined by HPLC
- CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
- Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
- CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
- The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
- Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
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Antigen: CD80 (B7-1)
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with no preservative; pH 7.2
Gene Alias: Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
Gene Symbols: CD80
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: CD80
Clone: 16-10A1
Applications: Flow Cytometry, Functional Assay, Neutralization
Conjugate: Functional Grade
Host Species: Armenian Hamster
Target Species: Canine, Mouse, Porcine
Gene Accession No.: Q00609
Gene ID (Entrez): 12519, 397161, 403765
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD80 (B7-1)
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with no preservative; pH 7.2
Gene Alias:
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
Gene Symbols:
CD80
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
CD80
Clone:
16-10A1
Applications:
Flow Cytometry, Functional Assay, Neutralization
Conjugate:
Functional Grade
Host Species:
Armenian Hamster
Target Species:
Canine, Mouse, Porcine
Gene Accession No.:
Q00609
Gene ID (Entrez):
12519, 397161, 403765
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD106 (VCAM-1)
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with no preservative; pH 7.2
Gene Alias: adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B
Gene Symbols: VCAM1
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: VCAM1
Clone: 429
Applications: Flow Cytometry, Functional Assay, Neutralization
Conjugate: Functional Grade
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P29533
Gene ID (Entrez): 22329
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD106 (VCAM-1)
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with no preservative; pH 7.2
Gene Alias:
adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B
Gene Symbols:
VCAM1
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
VCAM1
Clone:
429
Applications:
Flow Cytometry, Functional Assay, Neutralization
Conjugate:
Functional Grade
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P29533
Gene ID (Entrez):
22329
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD117 (c-Kit)
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with no preservative; pH 7.2
Gene Alias: __
Gene Symbols: __
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: __
Clone: ACK2
Applications: Flow Cytometry, Functional Assay
Conjugate: Functional Grade
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P05532
Gene ID (Entrez): 16590
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD117 (c-Kit)
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with no preservative; pH 7.2
Gene Alias:
__
Gene Symbols:
__
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
__
Clone:
ACK2
Applications:
Flow Cytometry, Functional Assay
Conjugate:
Functional Grade
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P05532
Gene ID (Entrez):
16590
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD120a (TNF Receptor I)
Concentration: 1 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with no preservative; pH 7.2
Gene Alias: CD120a; CD120a antigen; FPF; hypothetical protein; metalloproteinase inhibitor 4; MGC19588; MS5; n-smase activation assoc. factor; p55; p55 TNF receptor; p55-R; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TBP1; TBPI; TIMP metallopeptidase inhibitor 4; Timp4; TIMP-4; tissue inhibitor of metalloproteinase 4; tissue inhibitor of metalloproteinases 4; TNF receptor alpha chain; TNF receptor superfamily member 1A; TNF-alphaR1; TNF-alpha-R1; TNFalpha-R1; TNFAR; TNFR; TNF-R; TNFR I; TNFR1; TNF-R1; Tnfr-1; TNFR1-d2; Tnfr-2; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRp55; Tnfrsf1a; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor superfamily member 1A, membrane form; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor; Tumor necrosis factor-binding protein 1
Gene Symbols: Tnfrsf1a
Primary or Secondary: Primary
Content And Storage: 4° C
Gene: Tnfrsf1a
Clone: 55R-170
Applications: Functional Assay, Neutralization
Conjugate: Functional Grade
Host Species: Armenian Hamster
Target Species: Mouse
Gene Accession No.: P25118
Gene ID (Entrez): 21937
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD120a (TNF Receptor I)
Concentration:
1 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with no preservative; pH 7.2
Gene Alias:
CD120a; CD120a antigen; FPF; hypothetical protein; metalloproteinase inhibitor 4; MGC19588; MS5; n-smase activation assoc. factor; p55; p55 TNF receptor; p55-R; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TBP1; TBPI; TIMP metallopeptidase inhibitor 4; Timp4; TIMP-4; tissue inhibitor of metalloproteinase 4; tissue inhibitor of metalloproteinases 4; TNF receptor alpha chain; TNF receptor superfamily member 1A; TNF-alphaR1; TNF-alpha-R1; TNFalpha-R1; TNFAR; TNFR; TNF-R; TNFR I; TNFR1; TNF-R1; Tnfr-1; TNFR1-d2; Tnfr-2; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRp55; Tnfrsf1a; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor superfamily member 1A, membrane form; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor; Tumor necrosis factor-binding protein 1
Gene Symbols:
Tnfrsf1a
Primary or Secondary:
Primary
Content And Storage:
4° C
Gene:
Tnfrsf1a
Clone:
55R-170
Applications:
Functional Assay, Neutralization
Conjugate:
Functional Grade
Host Species:
Armenian Hamster
Target Species:
Mouse
Gene Accession No.:
P25118
Gene ID (Entrez):
21937
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody