CD226 (DNAM-1) Monoclonal Antibody (10E5), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: This 10E5 monoclonal antibody reacts with mouse CD226, which is also known as DNAX accessory molecule-1 (DNAM-1)
• CD226 is an adhesion molecule expressed primarily on T cells, including thymocytes
• The highest levels of CD226 are found on naive CD8 T cells
• Moreover, expression of CD226 is regulated differentially in CD4 T cells
• Under certain culture conditions, CD226 can be upregulated in Th1 and downregulated in Th2 and Th0 cells
• This cell surface receptor can also be detected on natural killer cells, some B cell subsets, monocytes, and platelets
• Interaction with its ligands, the poliovirus receptor (CD155) and CD112, results in adhesion and cell signaling events that promote innate and adaptive immunity, including differentiation, survival, and cytotoxicity
• The 10E5 monoclonal antibody has been reported to inhibit antigen-specific T cell proliferation and IFN-gamma production in vivo
• Applications Reported: This 10E5 antibody has been reported for use in flow cytometric analysis and functional assays
• Applications Tested: This 10E5 antibody has been tested by flow cytometric analysis of 3-day CD3/CD28-stimulated mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes
• DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells
• DNAM-1 is also expressed by a subset of CD3 positive thymocytes.