IL-1 beta Monoclonal Antibody (CRM56), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The CRM56 antibody reacts with human and baboon interleukin-1 beta; (IL-1 beta)
• IL-1 beta is a 17 kDa factor secreted primarily by monocytes
• IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors
• IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis
• Applications Reported: The CRM56 antibody has been reported for use in cytokine neutralization, and ELISA
• Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry
• Functional Grade purified CRM56 antibody, cat
• 16-7018, is recommended for use in functional assays
• Applications Tested: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1β (IL-1β) in combination with the biotin CRM57 (13-7016) antibody for detection and recombit human IL-1β (14-8018) as the standard
• A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL
• A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate
• The Functional Grade Purified CRM56 antibody has been tested by LAL assay to verify low endotoxin and by bioassay to verify neutralization of IL-1β bioactivity
• For in vitro neutralization, the CRM56 antibody at 2
• Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells
• IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol
• Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals
• Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta
• IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism
• Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions
• IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha
• IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition
• IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury
• Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.