CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein
• FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells
• The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes
• Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form
• Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended
• Applications Reported: NOK-1 has been reported for use in flow cytometric analysis
• NOK-1 has also been reported in blocking of FasL mediated killing in functional assays
• Applications Tested: The NOK-1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space
• Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family
• As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR
• CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD)
• The soluble protein can be released from cells upon cleavage by metalloproteinases
• Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins
• CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye
• CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney
• It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses
• CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis
• Various cells express FAS, where CD178 is expressed predominantly on activated T cells
• FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity
• CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension
• The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.