CD19 Monoclonal Antibody (6OMP31), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The 6OMP31 monoclonal antibody reacts with human, mouse, and rat CD19
• CD19 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily
• CD19 was first identified as the B4 antigen of human B lymphocytes using the anti-B4 monoclonal
• It is expressed by B cells during all stages of development, except on terminally differentiated plasma cells, and on follicular dendritic cells
• CD19 acts primarily as a B cell co-receptor and together with CD21, CD81, Leu13, and MHC class II forms a multi-molecular complex that associates with the BCR
• CD19 plays a role in the development, activation, differentiation, and proliferation of B cells
• CD19 deficiency leads to an overall impaired humoral immune response
• Mutations in CD19 have been associated with severe immunodeficiency syndromes
• Although it is unclear whether CD19 contributes directly to B cell carcinogenesis, it is expressed on most B cell tumors, such as acute lymphoblastic leukemias and B cell lymphomas
• The use of CD19 monoclonal antibodies for therapies against lymphoma, leukemia and autoimmune disorders are currently being explored
• Applications Reported: This 6OMP31 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections
• CD19 is a member of the immunoglobulin superfamily and has two Ig like domains
• The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains
• CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage
• Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen
• The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias
• Lymphocytes proliferate and differentiate in response to various concentrations of different antigens
• The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors
• CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation
• Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge
• Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.