7078474

CD45R (B220) Monoclonal Antibody (RA3-6B2), Super Bright™ 600, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD45R (B220)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias

B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen

Gene Symbols

PTPRC

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

PTPRC

Clone

RA3-6B2

Applications

Flow Cytometry

Conjugate

Super Bright 600

Host Species

Rat

Target Species

Human, Mouse

Gene Accession No.

P06800, P08575

Gene ID (Entrez)

19264, 5788

Isotype

IgG2a κ

Purification Method

Protein G

Product Type

Antibody

Description

  • Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule
  • CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells
  • However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen
  • Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
  • We recommend using a 610/20 bandpass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat
  • SB-4400) to minimize any non-specific polymer interactions
  • CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family
  • PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation
  • CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains
  • CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
  • CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains
  • Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
  • In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
  • Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

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Fischer Scientific

7078474

Rat Monoclonal Antibody...


Antigen:
CD45R (B220)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen

Gene Symbols:
PTPRC

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
PTPRC

Clone:
RA3-6B2

Applications:
Flow Cytometry

Conjugate:
Super Bright 600

Host Species:
Rat

Target Species:
Human, Mouse

Gene Accession No.:
P06800, P08575

Gene ID (Entrez):
19264, 5788

Isotype:
IgG2a κ

Purification Method:
Protein G

Product Type:
Antibody

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7078479

Rat Monoclonal Antibody...


Antigen:
CD8a

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein

Gene Symbols:
Cd8a, Cd8b1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd8a

Clone:
53-6.7

Applications:
Flow Cytometry

Conjugate:
Super Bright 600

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P01731, P10300

Gene ID (Entrez):
12525, 12526

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Fischer Scientific

7078480

Rat Monoclonal Antibody...


Antigen:
CD8a

Concentration:
0.5 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein

Gene Symbols:
Cd8a, Cd8b1

Primary or Secondary:
Primary

Content And Storage:
4° C

Gene:
Cd8a

Clone:
4SM16

Applications:
Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P01731, P10300

Gene ID (Entrez):
12525, 12526

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7078498

Mouse Monoclonal Antibody...


Antigen:
HLA-DR

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
AI323765; BLA-DRB3; BOLA-DRA; BoLA-DR-alpha; BoLA-DRB; BOLA-DRB3; BoLA-DRB3 protein; BoLA-DRB3.2; Bota-DRB01; Bota-DRB02; Bota-DRB04; Bota-DRB07; Bota-DRB21; Bota-DRB22; bovine leukocyte antigen; Cd74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule; Cd74 molecule, major histocompatibility complex, class II invariant chain; class II-associated invariant chain peptide; CLIP; Clone P2-beta-3; DASS-397D15.1; DHLAG; dinucleotide microsatellite; DLA class II histocompatibility antigen, DR-1 beta chain; DLA DRBB1 beta chain; DLA-DR beta; DLA-DRB1; DLA-DRBB1; DR beta-5; DR beta-chain antigen binding domain; DR-16; DR2-beta-2; DR4; DR-4; DR7; DR9; DR-9; DRB; DRB1; DRB1 transplantation antigen; DRB3; DRB4; DRBB1; DR-beta chain; DR-beta chain MHC class II; DRw10; Dw2; DW2.2/DR2.2; E-alpha-f; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; H-2 class II histocompatibility antigen gamma chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2Ea; H2-Ea; H2-Ea-ps; H2-IE-alpha; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility antigen HLA-DR alpha; histocompatibility complex, class II, DR beta 3; histocompatibility: class II antigens, gamma chain of; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1-15 beta chain; HLA class II histocompatibility antigen, DRB1-16 beta chain; HLA class II histocompatibility antigen, DRB1-3 chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB2; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLA-DR-gamma; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; ia antigen-associated invariant chain; Ia3; Ia-3; Ia-associated invariant chain; Ia-GAMMA; I-E alpha MHC class II; I-Ealpha; II; integral membrane glycoprotein; invariant gamma chain; invariant polypeptide of major histocompatibility complex, class II antigen-associated; INVG34; LA-DRB; leukocyte antigen; leukocyte antigen class II; leukocyte antigen DRB3; lymphocyte antigen DRB1; major histocompatibility complex class II DR-beta chain; major histocompatibility complex, class II, DR alpha; major histocompatibility complex, class II, DR alpha precursor; major histocompatibility complex, class II, DR beta 1; major histocompatibility complex, class II, DR beta 3; major histocompatibility complex, class II, DR beta 4; major histocompatibility complex, class II, DR beta 4 precursor; major histocompatibility complex, class II, DR beta 5; major histocompatibility complex, class II, DRB3; MHC cell surface glycoprotein; MHC class I antigen; MHC class II antigen; MHC class II antigen beta chain; MHC class II antigen BoLA-DRB3; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*15; MHC class II antigen DRB1*16; MHC class II antigen DRB1*3; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen E alpha; MHC class II antigen HLA-DR-beta; MHC class II DLA DRB1 beta chain; MHC class II DLA-DRB; MHC class II DLA-DR-beta-1; MHC class II DR beta 1; MHC class II DR beta chain; MHC class II DR beta-chain; MHC class II DR-ALPHA

Gene Symbols:
CD74, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
HLA-DRB4

Clone:
LN3

Applications:
Flow Cytometry

Conjugate:
Super Bright 600

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P01903, P01911, P04233, P13762, P79483, Q30154

Gene ID (Entrez):
3122, 3123, 3125, 3126, 3127, 972

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody