IgE Mouse anti-Human, Brilliant Violet 421, Clone: G7-26, BD

Description

• The G7-26 monoclonal antibody specifically recognizes the constant region of human Immunoglobulin E (IgE)
• It does not crossreact with other immunoglobulin heavy chain isotypes
• IgE exists in a transmembrane form that is expressed by B lymphocytes and serves as an antigen receptor
• Soluble IgE is produced and secreted by activated B cells and plasma cells
• IgE may bind through its constant region to cell surface receptors, such as the high-affinity ( ) or low-affinity ( /CD23) receptors for IgE
• Fc RI is expressed on mast cells, basophils, and at lower levels, on dendritic cells and monocytes
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.