CD209b (SIGN-R1) Monoclonal Antibody (eBio22D1 (22D1)), APC, eBioscience™, Invitrogen™

Description

• Description: The eBio22D1 monoclonal antibody reacts with mouse SIGNR1 (CD209b)
• SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN
• It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae
• It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS
• Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase
• The eBio22D1 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4
• Applications Reported: This 22D1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 22D1 antibody has been tested by flow cytometric analysis of young mice splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• CD209b (SIGN-R1) Monoclonal antibody specifically detects CD209b (SIGN-R1) in Mouse samples
• It is validated for Flow Cytometry.