CD267 (TACI) Monoclonal Antibody (ebio8F10-3), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody eBio8F10.3 recognizes TACI (Transmembrane Activator and Calcium-modulator and CAML Interactor) also known as CD267
• TACI is a type III membrane protein belonging to the TNF receptor superfamily
• The ligand for TACI has been shown to be BAFF (BLyS) and APRIL
• TACI is expressed on the surface of a subset of maturing splenic B cells of the transitional type termed T2
• Genetic studies have shown a role for TACI in both stimulation and inhibition of B cell proliferation
• This actual role for TACI in the mouse has still not been determined
• Recently human TACI has been shown to have repressive activities which inhibit BAFF-R function
• Applications Reported: This ebio8F10-3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This ebio8F10-3 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Members in the TNF superfamily regulate immune responses and induce apoptosis
• Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL-1/THANK/zTNF4 and April/TALL-2, respectively
• BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion
• April regulates immunological and non-immunological cell growth
• Three receptors, BCMA, TACI, and BAFF-R, for BAFF and April were recently identified
• TACI, like BCMA, binds BAFF and April
• TACI and its ligands regulate humoral immune responses, activate NF-kappa-B and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.