Podoplanin Monoclonal Antibody (NZ-1.3), APC, eBioscience™, Invitrogen™

Description

• Description: Podoplanin is a 40-kDa type I transmembrane sialomucin-like glycoprotein involved in platelet aggregation and cancer metastasis
• This protein is expressed on lymphatic endothelial cells and follicular dendritic cells, as well as on many human cancers, including squamous cell carcinoma, glioblastoma, seminoma, and mesothelioma
• Podoplanin expression has been demonstrated to be regulated by the Fos protein of the Activating Protein-1 (AP-1) transcription factor complex
• Much of its functionality arises via interaction with its endogenous receptor, C-type lectin-like receptor-2 (CLEC-2)
• This antibody has been reported to block the interaction between Podoplanin and CLEC-2
• Applications Reported: This NZ-1.3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This NZ-1.3 antibody has been pre-titrated and tested by flow cytometric analysis of U87 cell line
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight
• Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs
• Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells
• As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes
• Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential
• Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue
• Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells
• Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC
• Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm
• Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.