7090328

CD25 Monoclonal Antibody (CD25-4E3), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD25

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias

ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR

Gene Symbols

IL2RA

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

IL2RA

Clone

CD25-4E3

Applications

Flow Cytometry

Conjugate

PE-Cyanine7

Host Species

Mouse

Target Species

Human, Non-human Primate

Gene Accession No.

P01589

Gene ID (Entrez)

3559

Isotype

IgG2b κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha
  • CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages
  • This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor
  • IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes
  • CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes
  • This monoclonal antibody is cross-reactive to rhesus monkey
  • Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (0.125 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • CD25 Monoclonal antibody specifically detects CD25 in Human, Non-human Primate samples
  • It is validated for Flow Cytometry.

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Antigen:
CD25

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR

Gene Symbols:
IL2RA

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IL2RA

Clone:
CD25-4E3

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Mouse

Target Species:
Human, Non-human Primate

Gene Accession No.:
P01589

Gene ID (Entrez):
3559

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
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Concentration:
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Classification:
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Form:
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Regulatory Status:
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Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

Gene Symbols:
CD27

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD27

Clone:
LG.7F9

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Armenian Hamster

Target Species:
Human, Mouse, Rat

Gene Accession No.:
P26842, P41272

Gene ID (Entrez):
21940, 500318, 939

Isotype:
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Purification Method:
Affinity chromatography

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Form:
Liquid

Regulatory Status:
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Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
CD antigen 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

Gene Symbols:
CD27

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD27

Clone:
O323

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Mouse

Target Species:
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Gene Accession No.:
P26842

Gene ID (Entrez):
939

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Affinity chromatography

Product Type:
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Antigen:
CD31 (PECAM-1)

Concentration:
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Classification:
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Form:
Liquid

Regulatory Status:
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Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule

Gene Symbols:
Pecam1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Pecam1

Clone:
390

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q08481

Gene ID (Entrez):
18613

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody