CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family
• CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells
• CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood
• The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163
• Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10
• Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF alpha, can cause shedding of a soluble form of CD163, whose function is unclear
• Applications Reported: This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioGHI/61 (GHI/61) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• CD163 Monoclonal antibody specifically detects CD163 in Human samples
• It is validated for Flow Cytometry.