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7090440

OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

OVA257-264 (SIINFEKL) peptide bound to H-2Kb

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

Clone

eBio25-D1.16 (25-D1.16)

Applications

Flow Cytometry

Conjugate

PE-Cyanine7

Host Species

Mouse

Target Species

Mouse

Gene Alias

H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Description

  • Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide
  • This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo
  • Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
  • OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples
  • It is validated for Flow Cytometry.

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Fischer Scientific

7090440

Mouse Monoclonal Antibody...

Antigen:
OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
eBio25-D1.16 (25-D1.16)
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Mouse
Target Species:
Mouse
Gene Alias:
H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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Fischer Scientific

7090441

Rat Monoclonal Antibody...

Antigen:
MERTK
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
DS5MMER
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
c-Eyk; c-mer; c-mer proto-oncogene tyrosine kinase; Eyk; MER; MER proto-oncogene, tyrosine kinase; MER receptor tyrosine kinase; MERTK; MGC133349; nmf12; Nyk; Proto-oncogene c-Mer; proto-oncogene tyrosine-protein kinase MER; rdy; receptor tyrosine kinase MerTK; Receptor tyrosine tinase gene probably the gene for Rdy; retinal dystrophy; RP38; sMER; sMERTK; soluble MER; soluble MERTK; STK kinase; Tyro 12; Tyro12; Tyrosine-protein kinase Mer
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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7090442

Rat Monoclonal Antibody...

Antigen:
FOXP3
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
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Clone:
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Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Bovine, Canine, Feline, Mouse, Porcine, Rat
Gene Alias:
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!

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Mouse Monoclonal Antibody...

Antigen:
T-bet
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Clone:
eBio4B10 (4B10)
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Mouse
Target Species:
Human, Mouse, Rhesus Monkey
Gene Alias:
HGNC:11599; LOW QUALITY PROTEIN: T-box transcription factor TBX21; Tbet; T-bet; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; TBT1; TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM; transcription factor T-box 21
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!