7090472
Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine7, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Ly-6A/E (Sca-1)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Gene Symbols
Ly6a, Ly6e
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
D7
Applications
Flow Cytometry
Conjugate
PE-Cyanine7
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P05533, Q64253
Gene ID (Entrez)
110454, 17069
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Description
- Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins
- D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells
- Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others
- Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype
- The Ly-6 family is involved in regulation and function of T cell activation
- Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset
- Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.06 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family
- It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice
- Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC
- Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen
- Sca-1 may be involved in the regulation of B and T cell activation.
Compare Similar Items
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Antigen: Ly-6A/E (Sca-1)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Gene Symbols: Ly6a, Ly6e
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: D7
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P05533, Q64253
Gene ID (Entrez): 110454, 17069
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Ly-6A/E (Sca-1)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
9804; locus A/E; Locus AE; Ly6; Ly67; Ly6a; Ly-6A.2; Ly-6A.2/Ly-6E.1; Ly6A/E; Ly-6A/E; ly6a-e; Ly6e; Ly-6E; Ly-6E.1; lymphocyte antigen 6 complex; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6 complex, locus E; lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; RIG-E; Sca1; Sca-1; Sca-2; stem cell antigen 1; Stem cell antigen 2; TAP; T-cell-activating protein; thymic shared antigen 1; Tsa1; TSA-1
Gene Symbols:
Ly6a, Ly6e
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
D7
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P05533, Q64253
Gene ID (Entrez):
110454, 17069
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD274 (PD-L1, B7-H1)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols: Cd274
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: MIH1
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Mouse
Target Species: Human
Gene Accession No.: Q9NZQ7
Gene ID (Entrez): 29126
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD274 (PD-L1, B7-H1)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols:
Cd274
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
MIH1
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
Q9NZQ7
Gene ID (Entrez):
29126
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: IgD
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Gene Symbols: Ighd
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: 11-26c (11-26)
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P01881
Gene ID (Entrez): 380797
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
IgD
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Gene Symbols:
Ighd
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
11-26c (11-26)
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P01881
Gene ID (Entrez):
380797
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: IL-2
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Symbols: IL2
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: JES6-5H4
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P04351
Gene ID (Entrez): 16183
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
IL-2
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Symbols:
IL2
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
JES6-5H4
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P04351
Gene ID (Entrez):
16183
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody