7090589
CD15 Monoclonal Antibody (HI98), eFluor™ 615, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD15
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
FUTIV, Lewis X, SSEA-1
Gene Symbols
FUT4
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
FUT4
Clone
HI98
Applications
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Conjugate
eFluor 615
Host Species
Mouse
Target Species
Human
Gene Accession No.
P22083
Gene ID (Entrez)
2526
Isotype
IgM κ
Purification Method
Affinity chromatography
Product Type
Antibody
Description
- Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X
- This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes
- Monocytes express this structure at varying degree while lymphocytes are negative
- Applications Reported: This HI98 antibody has been reported for use in immunohistochemical staing of frozen and FFPE tissue sections (IHC-F, IHC-P), and immunocytochemical staining
- Applications Tested: This HI98 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human human kidney cortex (IHC-P) using high pH antigen retrieval at less than or equal to 5 μg/mL
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- This product has not been validated for flow cytometric analysis
- Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75)
- A standard Alexa Fluor™ 594 filter is acceptable
- Excitation: 595 nm; Emission: 615 nm
- Filtration: 0.2 μm post-manufacturing filtered
- CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g
- granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells
- There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x
- Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction
- This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells
- Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins
- CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
Compare Similar Items
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Antigen: CD15
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: FUTIV, Lewis X, SSEA-1
Gene Symbols: FUT4
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: FUT4
Clone: HI98
Applications: Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Conjugate: eFluor 615
Host Species: Mouse
Target Species: Human
Gene Accession No.: P22083
Gene ID (Entrez): 2526
Isotype: IgM κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD15
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
FUTIV, Lewis X, SSEA-1
Gene Symbols:
FUT4
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
FUT4
Clone:
HI98
Applications:
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Conjugate:
eFluor 615
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P22083
Gene ID (Entrez):
2526
Isotype:
IgM κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: LYVE1
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: 1200012G08Rik; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extra cellular link domain-containing 1; extracellular link domain containing 1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; HAR; hyaluronic acid receptor; lymphatic endothelial hyaluronan receptor LYVE-1; lymphatic vessel endothelial HA receptor-1; lymphatic vessel endothelial HA recptor-1; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; sLyve 1; sLyve1; soluble LYVE 1; soluble LYVE1; UNQ230/PRO263; XLKD1
Gene Symbols: Lyve1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Lyve1
Clone: ALY7
Applications: Immunocytochemistry, Immunohistochemistry (Frozen)
Conjugate: eFluor 615
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q8BHC0
Gene ID (Entrez): 114332
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
LYVE1
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
1200012G08Rik; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extra cellular link domain-containing 1; extracellular link domain containing 1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; HAR; hyaluronic acid receptor; lymphatic endothelial hyaluronan receptor LYVE-1; lymphatic vessel endothelial HA receptor-1; lymphatic vessel endothelial HA recptor-1; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; sLyve 1; sLyve1; soluble LYVE 1; soluble LYVE1; UNQ230/PRO263; XLKD1
Gene Symbols:
Lyve1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Lyve1
Clone:
ALY7
Applications:
Immunocytochemistry, Immunohistochemistry (Frozen)
Conjugate:
eFluor 615
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q8BHC0
Gene ID (Entrez):
114332
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Rat IgG2a kappa
Concentration: 0.2 mg/mL
Classification: __
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Gene Symbols: __
Primary or Secondary: Isotype Control (Primary)
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: __
Clone: eBR2a
Applications: Control, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
Conjugate: eFluor 615
Host Species: Rat
Target Species: Not Applicable
Gene Accession No.: __
Gene ID (Entrez): __
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: __
Antigen:
Rat IgG2a kappa
Concentration:
0.2 mg/mL
Classification:
__
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Gene Symbols:
__
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
__
Clone:
eBR2a
Applications:
Control, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
Conjugate:
eFluor 615
Host Species:
Rat
Target Species:
Not Applicable
Gene Accession No.:
__
Gene ID (Entrez):
__
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
__
Antigen: Ki-67
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
Gene Symbols: Mki67
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Mki67
Clone: SolA15
Applications: Immunocytochemistry, Immunohistochemistry (Frozen)
Conjugate: eFluor 615
Host Species: Rat
Target Species: Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat
Gene Accession No.: E9PVX6, P46013
Gene ID (Entrez): 100686578, 102135895, 17345, 291234, 4288
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Ki-67
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
Gene Symbols:
Mki67
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Mki67
Clone:
SolA15
Applications:
Immunocytochemistry, Immunohistochemistry (Frozen)
Conjugate:
eFluor 615
Host Species:
Rat
Target Species:
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat
Gene Accession No.:
E9PVX6, P46013
Gene ID (Entrez):
100686578, 102135895, 17345, 291234, 4288
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody