CD26 Monoclonal Antibody (H194-112 (H194-112.12.4.4.5)), PerCP-Cyanine5.5, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody H194-112 recognizes mouse CD26, a thymocyte-activating molecule (THAM)
• CD26 is a surface expressed and soluble ectoenzyme (dipeptidyl peptidase IV) that cleaves dipeptides from the amino terminus of proteins
• In particular CD26 has been shown to cleave CXCL12, CCL22, neuropeptide Y and glucagon
• CD26 is involved in adhesion, apoptosis, disease regulation, and engraftment
• Expression is regulated during thymic development at the messenger RNA level
• Additionally, CD26 protein is found on thymocytes, a percentage of resting B cells, stimulated B cells, NK cells and a percentage of resting and activated T cells and other epithelial cell types
• On human cells more CD26 protein is found on Th2 cells
• Functional CD26 is expressed on Sca-1+c-Kit+lin- cells
• This expression has been shown to correlate with mobilization of progenitor cells
• Inhibition of CD26 activity through a variety of compounds enhances allogenic stem cell homing and engraftment
• This antibody is reported to have activating function
• Applications Reported: This H194-112 (H194-112.12.4.4.5) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This H194-112 (H194-112.12.4.4.5) antibody has been tested by flow cytometric analysis of mouse splenocytes or thymocytes
• This can be used at less than or equal to 1 μg per test
• CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family
• CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation
• CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia
• CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection
• CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26
• Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides
• Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity
• CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts
• Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.