7090709

CD31 (PECAM-1) Monoclonal Antibody (390), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD31 (PECAM-1)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule

Gene Symbols

Pecam1

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Pecam1

Clone

390

Applications

Flow Cytometry

Conjugate

PerCP-eFluor 710

Host Species

Rat

Target Species

Mouse

Gene Accession No.

Q08481

Gene ID (Entrez)

18613

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa
  • This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets
  • It has been reported that CD38 binds to CD31
  • Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction
  • Applications Reported: This 390 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 390 antibody has been tested by flow cytometric analysis of mouse spleen and bone marrow cells
  • This can be used at less than or equal to 0.125 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
  • We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases
  • CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells
  • N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions
  • CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis
  • It is one of key regulatory molecules in vascular system.

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Fischer Scientific

7090709

Rat Monoclonal Antibody...


Antigen:
CD31 (PECAM-1)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule

Gene Symbols:
Pecam1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Pecam1

Clone:
390

Applications:
Flow Cytometry

Conjugate:
PerCP-eFluor 710

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q08481

Gene ID (Entrez):
18613

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Fischer Scientific

7090715

Rat Monoclonal Antibody...


Antigen:
CD38

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; Cd38; CD38 antigen; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase); CD38 antigen (p45); CD38 antigen p45; CD38 molecule; CD38H; Cd38-rs1; cluster of differentiation 38; cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; I-19; NAD(+) nucleosidase; NAD+ nucleosidase; NIM-R5 antigen; T10

Gene Symbols:
CD38

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD38

Clone:
90

Applications:
Flow Cytometry

Conjugate:
PerCP-eFluor 710

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P56528

Gene ID (Entrez):
12494

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Img

Fischer Scientific

7090716

Mouse Monoclonal Antibody...


Antigen:
CD38

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; Cd38; CD38 antigen; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase); CD38 antigen (p45); CD38 antigen p45; CD38 molecule; CD38H; Cd38-rs1; cluster of differentiation 38; cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; I-19; NAD(+) nucleosidase; NAD+ nucleosidase; NIM-R5 antigen; T10

Gene Symbols:
CD38

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD38

Clone:
HB7

Applications:
Flow Cytometry

Conjugate:
PerCP-eFluor 710

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P28907

Gene ID (Entrez):
952

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Fischer Scientific

7090720

Rat Monoclonal Antibody...


Antigen:
CD40

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AI326936; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; Cd40; CD40 antigen; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen, TNF receptor superfamily member 5; CD40 molecule; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; GP39; HIGM1; I79_006806; IGM; IMD3; Immunoglobulin M; ImmunoglobulinM; membrane protein CD40; MGC9013; p50; receptor for ligand CD154; sCD40; soluble CD40; T-BAM; T-cell differentiation antigen; Tnfrsf5; TRAP; tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

Gene Symbols:
CD40

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD40

Clone:
1C10

Applications:
Flow Cytometry

Conjugate:
PerCP-eFluor 710

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P27512

Gene ID (Entrez):
21939

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody