CD325 (N-Cadherin) Monoclonal Antibody (8C11), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin
• CD325 is a 130 kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain
• CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites
• Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis
• Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells
• Applications Reported: This 8C11 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 8C11 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cell line
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion
• Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species
• N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins
• The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin
• Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• The protein functions during gastrulation and is required for establishment of left-right asymmetry.