7090996

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), APC-eFluor™ 780, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD31 (PECAM-1)

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias

C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule

Gene Symbols

Pecam1

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Pecam1

Clone

WM-59 (WM59)

Applications

Flow Cytometry

Conjugate

APC-eFluor 780

Host Species

Mouse

Target Species

Human

Gene Accession No.

P16284

Gene ID (Entrez)

5175

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

Description

  • Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa
  • This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets
  • It has been reported that CD38 binds to CD31
  • Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction
  • Applications Reported: This WM-59 (WM59) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This WM-59 (WM59) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (0.125 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Light sensitivity: This tandem is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases
  • CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells
  • N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions
  • CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis
  • It is one of key regulatory molecules in vascular system.

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Fischer Scientific

7090996

Mouse Monoclonal Antibody...


Antigen:
CD31 (PECAM-1)

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule

Gene Symbols:
Pecam1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Pecam1

Clone:
WM-59 (WM59)

Applications:
Flow Cytometry

Conjugate:
APC-eFluor 780

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P16284

Gene ID (Entrez):
5175

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Fischer Scientific

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Mouse Monoclonal Antibody...


Antigen:
CD34

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
(E3-independent) E2 ubiquitin-conjugating enzyme R1; AU040960; CD34; CD34 antigen; CD34 molecule; CDC34; cell division cycle 34; cell division cycle 34 homolog; cluster designation 34; cluster of differentiation antigen 34; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1

Gene Symbols:
Cd34

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd34

Clone:
4H11

Applications:
Flow Cytometry

Conjugate:
APC-eFluor 780

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P28906

Gene ID (Entrez):
947

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Fischer Scientific

7090998

Mouse Monoclonal Antibody...


Antigen:
CD38

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; Cd38; CD38 antigen; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase); CD38 antigen (p45); CD38 antigen p45; CD38 molecule; CD38H; Cd38-rs1; cluster of differentiation 38; cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; I-19; NAD(+) nucleosidase; NAD+ nucleosidase; NIM-R5 antigen; T10

Gene Symbols:
CD38

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD38

Clone:
HIT2

Applications:
Flow Cytometry

Conjugate:
APC-eFluor 780

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P28907

Gene ID (Entrez):
952

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7091000

Rat Monoclonal Antibody...


Antigen:
CD44

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
AU023126; AW121933; AW146109; Bovine CD44; CD44; CD44 antigen; CD44 antigen-like protein; CD44 molecule; CD44 molecule (Indian blood group); CD44 protein; CD44 variant; CD44A; CD44-like protein; CDW44; cell surface glycoprotein CD44; Cell surface glycoprotein CD44 (hyaluronate binding protein); cell-surface glycoprotein; chondroitin sulfate proteoglycan 8; CSPG8; ECMRIII; ECMR-III; Epican; extracellular matrix receptor III; extracellular matrix receptor-III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; HERMES; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; IN; LHR; Ly-24; lymphocyte antigen 24; lymphocyte surface antigen precursor CD44; MC56; MDU2; MDU3; METAA; MIC4; PGP I; Pgp1; PGP-1; PGPI; PGP-I; Phagocytic glycoprotein 1; phagocytic glycoprotein I; putative Bos taurus CD44; RHAMM; sCD 44; sCD44; soluble CD 44; soluble CD44

Gene Symbols:
CD44

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD44

Clone:
IM7

Applications:
Flow Cytometry

Conjugate:
APC-eFluor 780

Host Species:
Rat

Target Species:
Human, Mouse

Gene Accession No.:
P15379, P16070

Gene ID (Entrez):
12505, 960

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody